RNA

Part:BBa_K4899001:Design

Designed by: Morten Pedersen Hauger   Group: iGEM23_UiOslo-Norway   (2023-10-04)


miniaturized CAS12f guide RNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

due to the largely untested nature of the highly edited guide RNA, secondary structure simulations were used to ensure that the chosen anti-repeat and other variable regions did not change the wider secondary structure


Source

the majority of the sequence came from a paper (https://doi.org/10.1016/j.molcel.2021.08.008) with us designing the anti-repeat region of the single guide RNA

References