RNA
Part:BBa_K4899001:Design
Designed by: Morten Pedersen Hauger Group: iGEM23_UiOslo-Norway (2023-10-04)
miniaturized CAS12f guide RNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
due to the largely untested nature of the highly edited guide RNA, secondary structure simulations were used to ensure that the chosen anti-repeat and other variable regions did not change the wider secondary structure
Source
the majority of the sequence came from a paper (https://doi.org/10.1016/j.molcel.2021.08.008) with us designing the anti-repeat region of the single guide RNA